Effects of Insulin and IGFS on Growth and Functional Differentiation in Primary Cultured Rabbit Kidney Proximal Tubule Cells -Growth and membrane transport-

• 영문명: Effects of Insulin and IGFS on Growth and Functional Differentiation in Primary Cultured Rabbit Kidney Proximal Tubule Cells -Growth and membrane transport-
• 발행기관: 대한생리학회
• 저자명: Han, Ho-Jae Park, Kwon-Moo
• 간행물 정보: 『대한생리학회지』제29권 제2호, 191~202쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1995.12.31

영문 초록

The purpose of this study was to compare effects of insulin and IGFs on growth, apical membrane enzyme activities and membrane transport systems of primary cultured rabbit kidney proximal tubule cells. Results were as follows: 1. Insulin and IGF-I produced significant growth stimulatory effects at 5 X 10^-10M. IGF-II(5 X 10 ^-10 M) did not stimulate significant cell growth. 2. Insulin stimulated the phosphorylation of a 97 KD protein. It was difficult to determine whether this band represents insulin and/or the IGF-I receptor. 3. The activities of apical membrane enzymes (alkaline phosphatase, leucine aminopeptidase, and {γ}-glutamyl transpeptidase) were observed to be diminished after the cells were placed in the culture environment. 4. The uptake of α-MG, Pi and Na was significantly increased in cells incubated with insulin or IGF-I, IGF-II had no effect on the uptake of these substrates. 5. Na-pump activity, as assayed by Rb uptake, was significantly increased in cells treated with insulin or IGFs. In conclusion, insulin and IGF-I exert stimulatory effects on growth and membrane transporter(glucose, Na, Pi, and Na-pump) activities in primary cultured rabbit kidney proximal tubule cells. IGF-II had no effect on cell growth and membrane transporter(glucose, Na and Pi) activities.

키워드

Kidney Insulin IGF Transporter