Identification of Adenosine 5 -Tetraphosphate in Rabbit Platelets and its Metabolism in Blood

• 영문명: Identification of Adenosine 5 -Tetraphosphate in Rabbit Platelets and its Metabolism in Blood
• 발행기관: 대한생리학회
• 저자명: Lee, Joong-Woo Jeon, Sang-Jun Kong, In-Deok Jeong, Seong-Woo
• 간행물 정보: 『대한생리학회지』제29권 제2호, 217~224쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1995.12.31

영문 초록

Adenosine 5 -tetraphosphate (ATPP) was identified and quantified in extracts of rabbit platelets by elution of extracts containing authentic adenosine 5 -tetraphosphate and comparison of retention time with nucleotide standards using high-performance liquid chromatography technique. The amount of adenosine 5 -tetraphosphate was 0.62 nmoles/10⁹ cells which was 62-fold lower than that of ATP but only 10-fold lower than that of ADP. During platelet aggregation induced by thrombin, adenosine 5 -tetraphosphate was released to a relatively high extent. The degradation rates and halflives of adenosine 5 -tetraphosphate were measured during incubation of platelets in whole blood, erythrocyte suspension and plasma, respectively. The results suggest that plasma contributes more than blood cells to the catabolism of adenosine 5 -tetraphosphate. The pattern of degradation indicates that ATPP may be degraded mainly to AMP by soluble enzymes in plasma and very slowly to ADP and/or AMP by ectoenzymes on blood cells such as erythrocyte. The nature of the enzymes responsible fer the degradation of adenosine 5 -tetraphosphate is yet to be identified.

키워드

Adenosine 5 -tetraphosphate Platelet Nucleotides ATP Thrombin