학술논문
Effect of Temperature on Dicarboxylate Transport in Plasma Membrane Vesicles of Rabbit Proximal Tubule
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- 영문명
- Effect of Temperature on Dicarboxylate Transport in Plasma Membrane Vesicles of Rabbit Proximal Tubule
- 발행기관
- 대한생리학회
- 저자명
- Han, Kyung-Moon Kim, Young-Hee Woo, Jae-Suk Kim, Yong-Keun
- 간행물 정보
- 『대한생리학회지』제27권 제2호, 227~232쪽, 전체 6쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 1993.12.31
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국문 초록
영문 초록
The temperature dependence of Na+-dependent succinate uptake was studied in brush border (BBMV) and basolateral (BLMV) membrane vesicles isolated from the rabbit kidney cortex. The succinate uptake was markedly altered by temperature in a similar fashion in both membranes. The temperature dependence was characterized by a nonlinear Arrhenius plot with a break point at 22 and 25℃ for BBMV and BLMV, respectively. The activation energy was 3.91 and 17.09 kcal/mole at above and below the break point respectively, far BBMV; 2.65 and 14.05 kcal/mole, respectively, for BLMV. When temperature increased f개m 20 to 35℃, the Vmax of succinate transport increased from 3.49±0.11 to 5.90±0.86 nmole/mg/5 sec for BBMV and from 2.86±0.25 to 3.63±0.32 nmole/mg/5 sec for BLMV, with no change in Km in both membranes. These results suggest that renal dicarboxylate transport is similarly sensitive to a change in membrane physical state in BBMV and BLMV.
목차
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