Inheritance and Phenotypic Stability of Homoeolog-Edited Mutants in Hexaploid Solanum nigrum

• 영문명: Inheritance and Phenotypic Stability of Homoeolog-Edited Mutants in Hexaploid Solanum nigrum
• 발행기관: 한국육종학회
• 저자명: Eun Song Lee Hye-young Kang Yu Seon Chae Soon Ju Park
• 간행물 정보: 『Plant breeding and biotechnology』Vol.13, 97~107쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2025.02.28

국문 초록

Polyploid plants, such as hexaploid Solanum nigrum, present new challenges and opportunities for trait improvement due to their complex genome structures and homoeol ogous gene interactions. Gene editing of homoeologs provides a powerful tool to inves tigate the inheritance and phenotypic effects of mutations in polyploid systems. Here, we investigated the inheritance and phenotypic traits of homoeolog-edited triple and double mutants in hexaploid S. nigrum. Triple mutant genotypes (sns and snsp) were used for an alyzing inheritance patterns through backcrossing and self-pollination, revealing in dependent segregation of homoeologous variants and consistent genotypic ratios across generations. Yield and related traits were evaluated in fixed double mutants (sns1 sns2, sns1 sns3, sns2 sns3) under greenhouse and open-field conditions. Open-field cultivation significantly enhanced yield, primarily through increased fruit weight per inflorescence, whereas greenhouse cultivation showed more modest improvements. Other traits such as plant weight and Brix values remained largely unchanged. Yield improvements were stable across F3 to F5 generations, with minimal genotype-specific variability. These findings highlight the potential of homoeolog-targeted editing in polyploid plants to achieve stable and environment-specific yield enhancements.

키워드

Hexaploid S. nigrum Inheritance Homoeolog editing Yield traits