CRISPR/Cas9-Mediated Improvement of Major Rice Variety TBR225 for Low Cadmium Accumulation

• 영문명: CRISPR/Cas9-Mediated Improvement of Major Rice Variety TBR225 for Low Cadmium Accumulation
• 발행기관: 한국육종학회
• 저자명: Phuong Duy Nguyen Van Thi Pham Xuan Hoi Pham Mai Quynh Le
• 간행물 정보: 『Plant breeding and biotechnology』Vol.13, 71~83쪽, 전체 13쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2025.02.28

국문 초록

Cadmium (Cd) contamination in rice poses significant health risks to consumers. This study aimed to reduce Cd accumulation in the elite Vietnamese rice variety TBR225 (TBR225) by editing the Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5) gene using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR- associated 9 (Cas9) technology. We successfully generated OsNRAMP5 knockout mutants through Agrobacterium-mediated transformation. Sequencing analysis revealed various mutation types, including deletions, insertions, and substitutions, all resulting in pre mature stop codons or frameshift mutations. Three homozygous, transgene-free mutant lines were selected for further analysis. These lines exhibited significantly reduced Cd accumulation in roots (78.4-84.5%), shoots (72.3-83.8%), and grains (50.5-66.0%) compared to wild-type plants when exposed to different Cd concentrations. Importantly, the OsNRAMP5 mutations did not adversely affect major agronomic traits, including growth time, plant height, tiller number, grain yield, or amylose content. Additionally, the accumulation of essential micronutrients such as Fe and Zn remained unaffected in the mutant lines. Our results demonstrate the potential of CRISPR/Cas9-mediated OsNRAMP5 editing as an effective strategy for developing low-Cd accumulating rice va rieties without compromising agronomic performance or nutritional quality. This ap proach could significantly contribute to enhancing food safety in regions facing Cd con tamination challenges.

키워드

Cadmium CRISPR/Cas9 Knock-out gene OsNRAMP5 TBR225