Big-h3내 RGD-motif의 유전자 변이 유도에 의한 유방암 세포의 성장 및 이동성에 미치는 영향

• 영문명: Site-Directed Mutagenesis of RGD-Motif in Big-h3 and its Effect on the Growth and Invasion of Breast Cancer Cells
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 김연향(Yeon Hyang Kim)
• 간행물 정보: 『한국산업기술융합학회 학술대회 논문집』2019년 후반기 학술대회 논문집, 131~136쪽, 전체 6쪽
• 주제분류: 공학 > 산업공학
• 파일형태: PDF
• 발행일자: 2019.10.25

영문 초록

Extracellular matrix(ECM) plays an important role in cell migration in pathological conditions such as inflammation and cancer. Big-h3 (also named transforming growth factor β induced protein, betaig-h3, Bigh3, TGF-b-induced protein h3, keratoepithelin, and RGD-CAP) is an secretory extracellular protein and includes YH and RGD motif, which interact with extracellular matrix. In this study, big-h3 and big-h3 fragment (big-h3-f, 182 amino acid.) containing RGD-motif were cloned. To determine whether RGD-motif would affect cell growth, RGD-motif with aspatic acid to RGE-motif with glutamic acid of wild-type big-h3 or big-h3-f was mutated by site-directed mutagenesis. Big-h3, big-h3-f, mutant big-h3, or mutant big-h3-f was transfected into human breast cancer cell line MCF-7 and their expression were confirmed by immunoblotting. Wild type big-h3 and big-h3-f containing RGD-motif led to increased cell growth and invasion, compared with mutant big-h3 and mutant big-h3-f containing RGE-motif. Thus, RGD-motif of big-h3 may play a major role in cell growth and invasion regulation in regard to cell-extracellular matrix interaction.

키워드

Extracell Matrix(ECM) 세포외기질 Big-h3 형질전환 성장요인 유도단백질 Site-Drected Mutagenesis 점돌연변이 RGD-Motif 아르기닌-글리신-아스파긴산 모티브 Cell Growth 세포성장