스테로이드 호르몬 중간체 생산을 위한 2단계 발효공정

• 영문명: Two-stage Fermentation Process for the Production of Steroid Hormone Intermediates
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 김명국(Myung-Kuk Kim)
• 간행물 정보: 『한국산업기술융합학회 학술대회 논문집』2016년 전반기 학술대회 논문집, 11~15쪽, 전체 5쪽
• 주제분류: 공학 > 산업공학
• 파일형태: PDF
• 발행일자: 2016.05.20

영문 초록

In order to derive the hormone intermediate biosynthesis from water miscible phytosterol as a substrate, two-stage fermentation was carried out. The first stage was for the oxidation and decomposition of the phytosterol side chain using Rhodococcus sp. and second stage fermentation was for the hormone intermediate biosynthesis using Mycobacterium sp. As a result, the two-stage batch fermentation showed 84mole% of a production yield, and 11.4g/L of maximum production respectively. On the other hand two-stage fed-batch fermentation, by intermittent fed of glucose, showed 85mole%, and 15g/L. This result is an increase of about 2.4 times greater than 35mole% of production yield of the two step process, including attempted others. So the present two-stage fermentation process would expect positive results for the production of steroid intermediates.

키워드

Two-stage fermentation Steroid hormone intermediates plant sterol