Streptococcus thermophilus KCTC14471BP의 안전성 평가 및 실크 피브로인 코팅의 면역 증진 효능

• 영문명: Safety Assessment and Immune Stimulating Effect of Streptococcus thermophilus KCTC14471BP Using Silk Fibroin Coating Treatment
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 권기철 허보혜 손성호 권유진 권미진 장주현 신혜정 김인수 박성엽 김경환 김병국 신창훈 김병용
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』8권 1호, 39~47쪽, 전체 9쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2022.06.30

영문 초록

Probiotic strain is known to regulate the immune system by colonizing in the intestine and interact with intestinal cell receptors of lymphoid tissue. In this study, safety of Streptococcus thermophilus KCTC14471BP and silk fibroin coating effects was evaluated with respect to mucin binding abilities and immune system modulation. S. thermophilus KCTC14471BP was coated with silk fibroin by adding 1% water-soluble calcium and 0.1% silk fibroin. S. thermophilus KCTC14471BP showed the high activities of leucine arylamidase and β-galactosidase. Regarding the antibiotic resistance tests, S. thermophilus KCTC14471BP was susceptible to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol. S. thermophilus KCTC14471BP coated with silk fibroin showed the improved mucin binding ability from 16.1% to 71.3% and was confirmed to have no cytotoxicity against RAW 264.7 macrophage. S. thermophilus KCTC14471BP coated with silk fibroin showed dose-dependently significant increases in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. These results suggested that S. thermophilus KCTC14471BP can be expected as a promising probiotic bacteria for applications to food industries such as fermentation or functional foods.

목차

서 론

재료 및 방법

결 과

고 찰

References

키워드

probiotic Streptococcus thermophilus silk fibroin coating pro-inflammatory activity