프로바이오틱스 안전성 평가를 위한 항생제 저항성 유전자의 생물정보학적 분석법 비교

• 영문명: Comparison of Bioinformatic Analysis System for Antibiotic Resistance Gene Detection and Probiotics Safety Evaluation
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 곽우리(Woori Kwak) 김병용(Byung-Yong Kim)
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』8권 1호, 32~38쪽, 전체 7쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2022.06.30

영문 초록

The detection of the genome-based antibiotic resistance gene is an essential analysis process for the purpose of verifying the safety of probiotic strains, including lactic acid bacteria. In this study, 4 analysis platforms (AMRFinderPlus, staramr, rgi, ABRicate) were used for cross-comparison of 782 genomes corresponding to 19 kinds of probiotic species notified as functional foods. As a result of the analysis, the relatively fewest number of antibiotic resistance genes were detected in strains belonging to the order Lactobacillales, and antibiotic resistance genes were detected in 322 genomes used in the case of 2 types of Enterococcus genus. In addition, the presence and type of antibiotic resistance gene detection showed a lot of difference even for the same genome sequence depending on the database and analysis algorithm used by the analysis platform. These results can be confused in evaluating the potential for transmission of antibiotic resistance genes inherent in specific lactic acid bacteria and predicting potential risks that may occur in the future. Accordingly, it is judged that the antibiotic resistance gene-related analysis criteria need to be established more clearly and specifically in the safety evaluation of probiotic bacteria.

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키워드

probitoics antibiotic resistance safety bioinformatics Enterococcus