Direct Regeneration of Lemon Balm ( Melissa oficinalis L.) Plants from Nodal Explants: A Sample Protocol for Mass Propagation

• 발행기관: 고려대학교 생명자원연구소
• 저자명: Aktar Hossain Jun Hyeok Kim Hojoung Lee
• 간행물 정보: 『생명자원연구』생명자원연구 제18권, 1~9쪽, 전체 9쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2010.12.30

국문 초록

We developed an efficient in vitro regeneration protocol for the valuable medicinal plant lemon balm ( Melissa officinalis L.) from single node explants. The highest regeneration frequency (100%) and maximum number of shoots were achieved with half-strength Murashige and Skoog (MS) media supplemented with 1 mg L -1 6-benzylaminopurine (BA) alone (2.82 and 9.8 shoots at three and six weeks, respectively) or in combination with 0.2 mg L -1 napthalen-eacetic acid (NAA) (2.73 and 8.13 shoots at three and six weeks, respectively). Maximum shoot elongation was obtained with media containing 0.2 mg L -1 BA alone or in combination with 0.2 mg l -1 NAA (2.26 cm and 2.31 cm, respectively) over a period of three weeks. Elongated shoots were rooted in MS media with or without rooting hormone. However, the best rooting, as measured over a period of two weeks and in terms of rooting percentage, root number, root length, and root, shoot and total dry weight of the plantlets, was obtained in media supplemented with 1 mg L -1 indole-3-butyric acid (IBA). The rooted plantlets were successfully transferred to soil and acclimated under greenhouse conditions.

목차

I. Introduction

II. Materials and Methods

III. Results and Discussions

IV. References

키워드

In vitro regeneration Melissa officinalis nodal explants rooting ability shoot proliferation