피부흡수 대체시험법의 조건설정을 통한 수용성, 지용성 물질의 투과 특성 연구

• 영문명: A Permeation Characteristics Study of Water- or Oil-soluble Substances through Condition Setting for the In Vitro Skin Absorption Method
• 발행기관: 한국환경보건학회
• 저자명: 서지은(Ji-Eun Seo) 이진호(Jinho Lee) 김배환(Bae-Hwan Kim)
• 간행물 정보: 『한국환경보건학회지』제43권 제1호, 77~86쪽, 전체 10쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2017.02.28

영문 초록

Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oilsoluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428. Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS. Results: The permeation rate increased dose-dependently, and the permeation orders were KeraSkin TM > hairless mouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials. Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as ‘fast’ and ‘moderate’ compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.

목차

I. 서 론

II. 재료 및 방법

III. 연구결과

IV. 고 찰

V. 결 론

키워드

Franz cell skin permeation alternative method condition setting