Development of stabilized somatic cell standard solutions for calibration of electronic instruments analyzing bovine raw milk

• 발행기관: 한국예방수의학회
• 저자명: Jin San Moon Keum-Chan Jang Suk-Chan Jung Yi-Seok Joo Ji-Ho Kim Sung-Hwan Wee Hye Cheong Koo Yong Ho Park
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.36, No.3, 114~120쪽, 전체 7쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 2012.09.30

영문 초록

The current standard solutions for somatic cells used for calibration of electronic somatic cell counts as reference material in raw milk are preserved with bronopol, boric acid, sodium azide, or potassium dichromate, and have a shelf-life of only up to 6 days at 4 ± 2℃. In the present study, a set of somatic cell standard solutions (SCSS) with a stability of 5 months for calibration of electronic instruments was developed. Somatic cells collected from cow’s milk and stored in a bulk tank at a dairy plant were treated with 10% formaldehyde in order to improve stability, and then separated by centrifugation. The resulting somatic cell suspension was preserved with glycerin, thimerosal, and dimethyl sulfoxide, and diluted in 3% processed skim milk solution ranging from 200,000～250,000 (low level), 350,000~450,000 (medium level), and 550,000～650,000 (high level) cells/㎖. Each SCSS was verified by direct microscope somatic cell counting (DMSCC), C-reader, and commercial standard samples. The average somatic cell count determined by DMSCC was 248, 214, 226 × 10 3 cells/㎖, 436, 382, 420 × 10 3 cells/㎖, and 612, 595, 609 × 10 3 cells/㎖. The coefficient of variation representing the repeatability of DMSCC decreased as the number of cells increased, and was <10.0% in almost all SCSS samples (range 4.6～7.1%). No statistically significant difference in somatic cell concentration was observed after storage at refrigeration temperature (2~6℃) over a period of 22 weeks (5 months). The stabilized SCSS may be useful as a reference material for determination of somatic cell count and quality control in testing of bovine raw milk.

목차

Introduction

Materials and Methods

Results and Discussion

키워드

Bovine Milk Somatic Cell Count Reference Material