Effect of coenzyme Q10 via nitric oxide production and growth arrest of human colon cancer HCT116 cells

• 영문명: Effect of coenzyme Q10 via nitric oxide production and growth arrest of human colon cancer HCT116 cells
• 발행기관: 한국예방수의학회
• 저자명: Soojin Jang Jooyeon Lee Se Min Ryu Hanbyeol Lee Jeong-Ran Park Hyejin Kim Dongwook Kim Aera Jang Se-
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.41, No.2, 59~65쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2017.06.30

영문 초록

Colorectal cancer is a major cause of morbidity and mortality that accounts for over 9% of all incidences of cancer. Additionally, colorectal cancer is widely recognized as an environmental disease related to ill-defined cultural, social and lifestyle factors including physical activity, obesity, cigarette smoking and heavy alcohol consumption. Accordingly, natural phytochemicals and extracts have attracted attention because of their beneficial biological effects. Coenzyme Q10 (CoQ10) is a common supplementary medicine applied to increase bioenergetic capacity in various diseases. Therefore, in this study, we investigated whether CoQ10 treatment has any inhibitory effects and its related cellular mechanisms in human colon cancer HCT116 cells. A MTT assay revealed that CoQ10 slightly decreased the proliferation of HCT116 cells; however, glutathione- and superoxide dismutase- activity were unchanged in response to CoQ10 treatment. A DCF-DA assay revealed that CoQ10 slightly increased ROS release of HCT116 cells. However, in a nitric oxide (NO) assay, CoQ10 significantly increased NO production in a dose-dependent manner. The results of western blot analysis revealed that the protein levels of Bax, p21 and p53 were increased, whereas the protein level of Bcl2 was decreased suggesting that the CoQ10-mediated inhibitory mechanism is associated with apoptotic signaling. Taken together, our findings indicate that CoQ10 has an inhibitory effect on the growth of colon cancer cells via NO production that is associated with regulation of factors involved in apoptotic signaling including Bax, Bcl2, p21 and p53.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

키워드

Coenzyme Q10, colorectal cancer, nitric oxide, apoptosis