Simple Sequence Repeat (SSR) 마커를 이용한 장미 품종 식별

• 영문명: Identification of Rose (Rosa x hybrida) Varieties Using Simple Sequence Repeat (SSR) Markers
• 발행기관: 한국육종학회
• 저자명: 홍지화(Jee-Hwa Hong) 권용삼(Yong-Sham Kwon) 서정남(Jung-Nam Suh) 최근진(Keun-Jin Choi)
• 간행물 정보: 『한국육종학회지』Vol.45 No.2, 96~103쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.06.30

영문 초록

The objective of this study was to evaluate the suitability of simple sequence repeat markers for variety identification in 69 rose (Rosa x hybrida) varieties. A set of 112 SSR primer pairs was evaluated and 43 primer pairs showed polymorphism in 12 varieties. Twenty-two primer pairs out of 43 primer pairs showed high levels of polymorphism and reproducibility. The genetic relationship of 69 varieties was analyzed based on the marker genotypes of 22 SSRs. A total of 114 polymorphic amplified fragments were obtained by using 22 SSR markers. Two to ten SSR alleles were detected for each locus with an average of 5.18 alleles per locus. Average polymorphism information content (PIC) was 0.621, ranging from 0.211 to 0.813. A total of 114 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using unweighted pair-group method with arithmetical average (UPGMA). Cluster analysis of genetic diversity revealed that these SSR marker sets identified each genotypes of 69 rose varieties. These SSR markers may be used for wide range of practical application in variety identification of rose.

목차

서 언

재료 및 방법

결과 및 고찰

적 요

인 용 문 헌

키워드

Rose SSR marker Variety identification