토마토 숙성저해 유전자의 SCAR 분자표지 개발

• 영문명: A SCAR Marker Linked to RIPENING-INHIBITOR in Tomato
• 발행기관: 한국육종학회
• 저자명: 김현정(Hyoun-Joung Kim) 이흥렬(Heung-Ryul Lee) 현지영(Ji Young Hyun) 홍동오(Dong Oh Hong) 원동찬(Dong-Chan Won) 한지학(Chee Hark Harn)
• 간행물 정보: 『한국육종학회지』Vol.45 No.2, 104~108쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.06.30

영문 초록

Tomato shelf-life is important for its fresh market usage especially for exporting and transporting tomato in tropical climate. To increase the shelf-life, delaying maturity is one of the powerful methods. A ripening gene related to the fruit maturity delaying was previously known through tomato mutation. RIPENING-INHIBITOR (Rin) from the recessive mutation effectively blocks the ripening process, and when the hybrid (Rin/rin) was formed it shows slow-ripening and long shelf-life. Since breeders need the long shelf-life character in the tomato breeding program, we had set out a series of experiments to develop a Rin related DNA marker. We searched for the proper primer sequences in the deletion region between Rin and rin mRNA sequences, and also in the intron region of rin. Primers that simultaneously amplified two bands with a co-dominant pattern were selected and converted to the Sequence Characterized Amplified Region (SCAR) marker. As a preliminary data, the SCAR marker clearly distinguished genotypes between heterozygote (Rin/rin) and homozygote (Rin/Rin, rin/rin), and this would help select the tomato lines that have a characteristic of long shelf-life.

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키워드

Rin Ripening Shelf-life SCAR marker Tomato