사람의 매복치 치배낭에 잔존된 법랑상피의 종양성 변화를 탐지하기 위한 면역조직화학적 연구

• 영문명: IMMUNOHISTOCHEMICAL STUDY FOR THE DETECTION OF THE TUMOROUS CHANGES OF REMAIND ENAMEL EPITHELIUM IN THE IMPACTED HUMAN TOOTH FOLLICLE
• 발행기관: 대한구강악안면외과학회
• 저자명: 박영욱(Young Wook Park) 김지혁(Ji Hyuck Kim) 이석근(Suk Keun Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제25권 제3호, 216~224쪽, 전체 9쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1999.07.30

영문 초록

In order to investigate the potential cellular activity of remaind enamel epithelium of impacted tooth follicle, we have examined 75 tooth follicular tissues from impacted teeth by immunohistochemical methods. Particular focus on the proliferation, differentiation and apoptosis-antiapoptosis of remaind enamel epithelium was made. Follicular tissues were removed from impacted teeth, fixed in neutral formalin and prepared for 4㎛ thick 20 serial sections. Hematoxylin & eosin staining was done routinely, and the mucopolycharide materials of myxoid odontogenic mesenchyme was detected by histochemical reactions of toluidin blue, PAS, Van Gieson, Masson s trichrome Various antibodies, i.e., PCNA(proliferating cell nuclear antigen) and Ki-67 for proliferative activity, transglutaminase-C, transglutaminase-K, transglutaminase-E, TGF-β₁, bcl-2, and p53 were used. Apop-tag staining was also done to detect the phenomenon of apoptosis. Both of the reduced enamel epithelium in the luminal side of tooth follicle and the enamel epithelial rests scattered in the wall of tooth follicle showed frequent positive reaction for the PCNA and Ki-67, and these cells were also positive for the transglutaminase-C, K, E. On the other hand the enamel epithelium was not stained by Apop-tag staining but weakly positive for bcl-2 and p53. Relatively high amount of myxoid odontogenic mesenchyme was also diffusely observed in the tooth folliclular tissue, and the distribution of the myxoid odontogenic mesenchyme was closely related with the distribution of enamel epithelial rest infiltration. Taken together, these data may suggest that the remaind enamel epithelial cells are biologically acitive rather than the dormant state after the completion of tooth formation, and that the remaind enamel epithelium may has interaction with odontogenic mesenchyme and will not be degraded easily but may have a potential for the odontogenic tumors.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구재료 및 방법

Ⅲ. 연구결과

Ⅳ. 총괄 및 고안

Ⅴ. 결론

참고문헌

키워드

Remained enamel epithelium Tooth follicle Cellular activity Tumorous changes