사람 망막아세포종 세포에 발현된 P2Y11 수용체의 확인

• 영문명: Identification of P2Y11 Receptor Expressed in Human Retinoblastoma Cells
• 발행기관: 대한안과학회
• 저자명: 김윤희 김대란 전고이 이종혁 공인덕,Yoon Hee Kim, M.D., Dae Ran Kim, Ph.D., Ko I Chun, M.D., Jong Hyuck Lee, M.D., In Deok Kong, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number8, 1134~1142쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.08.31

영문 초록

Purpose: The present study aimed to identify the characteristics and physiological function of the P2Y11 receptor, a receptor likely expressed in human retinoblastoma cells. Methods: We measured possible P2Y11 signaling in WERI-Rb-1 cells using a Ca2+ imaging technique and RT-PCR. Results: 1) 10 ?M ATP elicited a strong but transient increase in Ca2+ in the WERI-Rb-1 cells, and this Ca2+ rise was well maintained after external Ca2+-depletion. 2) ATP-induced Ca2+ response arose entirely through Ca2+ mobilization. 3) P2Y11 agonist (BzATP, 100 ?M) increased Ca2+ by 31.2±3.7 % of ATP effect. 4) mRNA for P2Y11 subtype was identified using RT-PCR. Conclusions: P2Y11 purinergic activation can increase the intracellular calcium level through calcium mobilization in undifferentiated retinoblastoma cells, which may play an important role in cell proliferation, differentiation, and even pathologic processes.

키워드

Calcium, P2Y11 receptor, Retinoblastoma cell