상엽 에탄올가용분획의 글루코스전달체, acetyl-CoA 카복시라제 및 렙틴 mRNA 발현에 미치는 영향

• 영문명: Effects of Mori Folium Ethanol Soluble Fraction on mRNA Expression of glucose transporters, acetyl-CoA carboxylase and leptin
• 발행기관: 대한약학회
• 저자명: 류정화(Jeong Wha Ryu) 육창수(Chang Soo Yook) 정성현(Sung Hyun Chung)
• 간행물 정보: 『약학회지』제42권 제6호 (1998년), 589~597쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.12.31

영문 초록

Effects of Mori Folium Ethanol Soluble Fraction (MFESF) on mRNA expression of glucose transporters, acetyl-CoA carboxylase (ACC) and leptin were examined in db/db mice. 500 and 1000mg/kg dose for MFESF (designated by SY 500 and SY 1000, respectively) and 5mg/kg dose for acarbose were administered for 6 weeks. Quantitations of glucose transporters (GLUT-2 and GLUT-4), ACC and leptin mRNA were performed by RT-PCR and in vitro transcription with co-amplification of rat beta-actin gene as an internal standard. Muscular GLUT-4 mRNA expression in MFESF-treated groups were increased dose dependently. On the other hand, MFESF caused the GLLT-4 and leptin mRNA expressions in adipose tissue to decrease dose dependently, which means that triglyceride synthesis in adipocytes might be decreased and consequently signals adipocytes to inhibit the synthesis and release of leptin. Hepatic ACC mRNA expression in MFESF-treated groups was also decreased. and this may result in lowering of serum triiglyceride level. In contrast, liver GLUT-2 mRNA expressions in MFESF-treated and acarbose groups were increased. Higher rate of glucose uptake into hepatocytes is known to inhibit a phosphoenolpyruvate carboxykinase (PEPCK)-catalyzed reaction, which is a rate-limiting step in gluconeogenesis.

키워드

Mori Folium db/db mouse GLUT-2 GLUT-4 acetyl-CoA carboxylase leptin RT-PCR in vitro transcription.