크로마토그래프법에 의한 Hippuran131-I의 순도시험

• 영문명: Purity Test of Hippuran-131I by Chromatography
• 발행기관: 대한약학회
• 저자명: 김순옥(Soon Ok Kim)
• 간행물 정보: 『약학회지』제21권 제2호 (1977년), 110~114쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1977.06.30

영문 초록

The purified o-iodohippuric acid-131I, o-iodobenzoic acid-131I, sodium iodide-131I and mixtures of those compounds were applied to radio paper or radio thin layer chromatography to select the developing solvents with useful separation efficiency. The separation efficiencies were checked by radiochromatogram scannings. It has been found that the two dimensional radio paper chromatography using the solvent systems of 8% phenol and butanol-glacial acetic acid-water (4 : 1 : 1 v/v) and the radio thin layer chromatography using alumina gel plate and butanol-glacial acetic acid-water (4 : 1 : I v/v) are both efficient. In this method, the Rf value of o-iodohippuric acid-131I, o-iodobenzoic acid-131I and the unbound 131I is 0.48, 0.85 and 0.15, respectively.