상백피가 Substance P에 의한 비만세포 탈과립, 백혈구 침윤 및 혈관 투과성에 미치는 영향

• 영문명: Inhibitory Effect of Cortex mori on the Substance P-induced Mast Cell Degranulation, Granular Leukocyte Infiltraion, and Vascular Permeability in vivo
• 발행기관: 대한해부학회
• 저자명: 채옥희(Ok-Hee Chai) 정승호(Sung-Ho Jung) 이무삼(Moo-Sam Lee)
• 간행물 정보: 『대한해부학회지』제31권 제1호, 53~61쪽, 전체 9쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1998.02.28

국문 초록

상백피가 substance P (SP) 펩타이들에 의한 생쥐 피하조직내 백혈구의 침윤, 비만세포의 탈과립 및 흰쥐 피부반응을 통한 혈관 투과성에 어떠한 영향을 미치는 지를 알아본 결과 다음과 같은 결론을 얻었다. 1. SP 및 N-terminal peptide SP1-9는 생쥐 피하조직내로의 백혈구 침윤과 비만세포 탈과립을 용량의존성으로 증가시켰 다. 2. C-terminal peptide SP6-11은 백혈구 침윤과 비만세포의 탈과립을 유도하지 않았고, 내피세포의 혈관투과성을 증가시켰으며, 또한 N-terminal peptide SP1-9에 의한 비만세포 탈과립의 증가를 수반하지 않고 백혈구 침윤을 증가시켰다. 3. 상백피는 SP에 의한 백혈구 침윤을 거의 억제시키지 못하였으나, SP에 의한 비만세포의 탈과립과 N-terminal peptide SP1-9에 의하여 유도되는 백혈구의 침윤 및 비만세포의 탈과립을 유의성있게 억제시켰다. 4. 상백피는 N-terminal peptide SP1-9에 의한 피부반응 (혈관투과성)을 억제시켰으나, C-terminal peptide SP6-11에 의한 피부반응은 억제시키지 못하였다. 이상의 실험 결과로 보아 상백피는 SP에 의한 신경 염증성 반응을 일부 억제시키는 것으로 생각되는데, 이러한 억제현상 은 상백피가 혈관 투과성에 영향을 미치기보다는 비만세포를 안정화 시켜 일어나는 현상으로 생각된다.

영문 초록

Cortex mori (Morus alba L.), the root bark of mulberry tree, has been used as an antiphlogistic, diuretic, and expectorant in herbal medicine. It has been reported tha hot water extract of Cortex mori has an inhibitory effect on substance P- and compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells. Substance P (SP), a decapeptide which is present in sensory neurons, is believed to be a major mediator of neurogenic inflammations. N-terminal peptide of SP causes mast cell degranulation and its C-terminal peptide directly activates vascular endothelial cells to increase the vascular permeability. It has recently been shown that SP induces leukocyte infiltration in the skin, which is mediated through mast cell degranulation. The purpose of this study was to investigate whether Cortex mori could inhibit SP-induced leukocyte infiltration in mouse skin. Subcutaneous administration of SP (10-8 to 10-5 M) induced leukocytes infiltration in the skin of BALB/c mice 8h after the injection. Leukocyte infiltration of mouse skin was associated with mast cell degranulation which was induced by SP1-9 (10-7 to 10-5 M), but not by SP6-11 (10-7 to 10-5 M) which was found to increase the vascular permeability of endothelial cells in mouse skin. However, SP6-11 (10-7 to 10-5 M) enhanced SP1-9-induced leukocyte infiltration in the skin without any significant increase in mast cell degranulation. Cortex mori (10mg/ml) inhibited SP- and SP1-9-induced mast cell degranulation, but did not inhibite SP6-11-induced increase in vascular perameability. Taken together, the data indicate that the hot water extract of Cortex mori contains some substances with an activity to inhibit SPinduced leukocyte infiltration in to the mous skin. These activity of Cortex mori is likely due to the inhibition of release of chemical mediators from the mast cells which participate in neurogenic inflammations.

키워드

상백피 substance P 비만세포 탈과립 백혈구 침윤 혈관 투과성 신경 염증반응 Cortex mori Substance P Mast cell degranulation Leukocyte infiltration Vascular permeability Neurogenic inflammation