Coenzyme A transferase Fn1856-Fn1857 from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis

• 영문명: Coenzyme A transferase Fn1856-Fn1857 from Fusobacterium nucleatum: purification, crystallization, and X-ray crystallographic analysis
• 발행기관: 한국구조생물학회
• 저자명: Zhidan Zhao Xue Bai Wanjun Chen Dan Meng Yongbin Xu
• 간행물 정보: 『Biodesign』Vol 12, No 2, Jun, 23~28쪽, 전체 6쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2024.06.30

국문 초록

Fusobacterium nucleatum (F. nucleatum) is one of the most abundant gram-negative anaerobic bacteria, which has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer (CRC). In F. nucleatum, both the CoA transferases (Fn1856, Fn1857) play a crucial role in the butyrate metabolism pathway. Through investigation, the formation of complexes involves the execution of the functions of Fn1856 and Fn1857. In this study, the Fn1856-Fn1857 complex was successfully co-expressed and purified using Ni-NTA affinity and gel-filtration chromatography and then crystallized. The crystal diffracted to 2.80 Å resolution and belonged to the space group P65, with unit cell parameters of a = 166.518 Å, b = 166.518 Å, and c = 191.377 Å, α = β = 90.00° and γ = 120°. The asymmetric unit contained approximately eight monomers of Fn1856-Fn1857.

목차

INTRODUCTION

RESULTS AND DISCUSSION

METHODS

FUNDING

ACKNOWLEDGEMENTS

CONFLICT OF INTEREST

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