특수환경으로부터 세포외 지방분해효소를 생산하는 바실러스 서브틸리스 균주의 분리 및 특성에 관한 연구

• 영문명: A Study on the Isolation and Characterization of Bacillus subtilis Strains with Extracellular Lipase Production from Specific Environments
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 간밧 다리마 김민경 박보현 이용직 남개원 조석철 이상재
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』9권 1호, 25~30쪽, 전체 6쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

Screening of Bacillus subtilis strains capable of producing extracellular hydrolases, including lipolytic enzyme was carried out. Within the scope of this study, total 61 strains were isolated from various domestic specific environmental samples such as seawater, soil, Jeju ranch, hot spring, salt farm, and fermented food and identified as Bacillus subtilis based on 16S rRNA sequences. Among the isolates, 4 strains had extracellular lipase activity. The growth profile of the strains revealed that 4 strains showed well growth at 50℃, 3 strains at 55℃, and pH 7.0. All strains could tolerate salinity up to 5%(w/v), 3 strains up to 10%(w/v), and only one strain showed growth at 15%(w/v). Additionally, amylolytic and proteolytic activities were detected in these strains. The highest lipolytic, amylolytic, and proteolytic activity was detected in Bacillus subtilis YS-YR 5A. These results demonstrate the potential application of extracellular hydrolase-producing Bacillus subtilis strains, especially strain YS-YR 5A, as fermentation starter to enhance the functionality and multiplication of functional natural products in the food/medicine/cosmetics/bio industries.

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키워드

probiotic Bacillus subtilis isolation characterization extracellular lipase