김치 유래 Lactiplantibacillus plantarum THK-j112의 Streptococcus mutans에 대한 항균효과 및 항생물막 효과

• 영문명: Antimicrobial and Antibiofilm Effects of Lactiplantibacillus plantarum THK-j112 Isolated from Kimchi against Streptococcus mutans
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 유두나 이은지 정제용 이태후 국무창
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』9권 1호, 9~17쪽, 전체 9쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

Dental caries is an infectious disease accompanied by the destruction of teeth. It is a multifactorial disease caused by the interaction between microbes in dental plaque and food, with cariogenic Streptococcus mutans being one of the primary causes. In this study, the antimicrobial and antibiofilm activities of Lactiplantibacillus plantarum THK-j112 isolated from Kimchi, a traditional fermented food, against S. mutans were investigated. Furthermore, this paper also showed the possibility of developing this strain. After analyzing the 16S rRNA sequence, strain THK-j112 was named L. plantarum THK-j112 as it shared 99.93% similarity with L. plantarum ATCC 14917T. Both L. plantarum THK-j112 and L. plantarum ATCC 14917T demonstrated MIC values of 5 mg/mL and MBC values of 10 mg/mL against S. mutans KACC 16833T. The study confirmed that L. plantarum THK-j112 exhibited significant concentration-dependent inhibitory effects on biofilm formation, acid production, and glucosyltransferases(GTFs) production. Regarding hemolytic activity, Bacillus cereus ATCC 14579T, used as a positive control, was confirmed to cause hemolysis, whereas L. plantarum THK-j112 did not cause this phenomenon. Additionally, except for gentamicin, the antibiotic resistance test revealed a lower value than the MIC cut-off value for each antibiotic, indicating no resistance. Based on the findings, L. plantarum THK-j112 is expected to be useful in the food industry, such as functional food, as a lactic acid bacterium with antimicrobial and antibiofilm effects.

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키워드

antimicrobial antibiofilm Kimchi Streptococcus mutans Lactiplantibacillus plantarum THK-j112