Cryo-Electron Microscopy Research Center at Pusan National University

• 영문명: Cryo-Electron Microscopy Research Center at Pusan National University
• 발행기관: 한국구조생물학회
• 저자명: Bokyeong Park Sujin Lee Yeontae Jang Seu-Na Lee Seungmi Kim Jae-Sung Woo Kyung Lib Jang
• 간행물 정보: 『Biodesign』Vol 10, No 4, Dec, 79~85쪽, 전체 7쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2022.12.31

영문 초록

Growing demands for the structure determination of biological macromolecules using cryogenic electron microscopy (cryo-EM) necessitate expansion of national research infrastructure. In particular, better accessibility to transmission electron microscopes (TEMs) is needed for sample screening and high-resolution data collection as well as for newly emerging cryo-electron tomography (cryo-ET) analysis. Here we introduce recently established Cryo-EM Research Center at Pusan National University. The center is equipped with high-end TEM for data collection, lower-end TEM for sample screening and focused ion beam-scanning electron microscope (FIB-SEM) for sample milling at cryogenic temperature. Ancillary devices for sample vitrification and computational resources are also available for routine cryo- EM workflow. The center is anticipated to contribute to the development of structural biology field in Korea.

목차

INTRODUCTION

FACILITY

MICROSCOPES

ACCESSORY DEVICES

COMPUTING SYSTEM

CONCLUSION

REFERENCES