Fabrication of enzymatic biosensor based on the poly(3-thiophenecarboxylic acid-co-thiophene) polymer as electron-transfer materials

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Soo-Yeoun Kim Hyeon-Jin Jo Seong-Ho Choi
• 간행물 정보: 『한국응용과학기술학회지』한국응용과학기술학회지 제36권 제1호, 269~278쪽, 전체 10쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2019.02.28

영문 초록

We fabricated glucose oxidase (GOx)-modified biosensor for detection of glucose by physical immobilization of GOx after electrochemical polymerization of the conductive mixture monomers of the 3-thiophenecarboxylic acid (TCA) and thiophene (Th) onto ITO electrode in this study. We confirmed the successfully fabrication of GOx-modified biosensor via FT-IR spectroscopy, SEM, contact angle, and cyclic voltammetry. The fabricated biosensor has the detection limit of 0.1 μM, the linearity of 0.001-27 mM, and sensitivity of 38.75 mAM-1cm-2, respectively. The fabricated biosensor exhibits high interference effects to dopamine, ascorbic acid, and L-cysteine, respectively. From these results, the fabricated GOx-modified biosensor with long linearity and high sensitivity could be used as glucose sensor in human blood sample.

목차

1. Introduction

2. Experiments

3. Results and Discussion

4. Conclusions

키워드

Immobilization of glucose oxidase Electrochemical polymerization3-thiophenecarboxylic acid thiophene Electron transfer Human blood sample