The Evaluation on the Effectiveness as a Cosmetic Material of Ascidian shell Extract Using Zebrafish model

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Sin-Ho Park Bo-Ae Kim Jae-Chan Yang
• 간행물 정보: 『한국응용과학기술학회지』한국응용과학기술학회지 제36권 제1호, 258~268쪽, 전체 11쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2019.02.28

영문 초록

The extracts of AS contain in alloxanthin, halocynthiaxanthin, astaxanthin and 13 kinds of carotenoids. The aim of the study was to assess the anti-oxidant activity and cell viability of AS. The anti-oxidant activity was determined by using DPPH radical inhibition activity and superoxide dismutase (SOD)-like activity. The results of cell viability assay showed that the extracts from AS were cytotoxic at concentrations above 5.0 ㎎/㎖. This study was designed to examine inflammation induced by LPS, protection effect by UVB and the toxicity of Ascidian shell extract(ASE) as a functional cosmetic ingredient. Evaluation of embryo toxicity resulted in embryo coagulation and mortality when treated at 5.0, 10.0, 20.0 ㎎/㎖. At the lowest concentration of 1.0 ㎎/㎖, hatchability resulted in 100.0 % rate. The results of arrhythmia measurement in larvae showed similarity to the evaluation of embryo toxicity. This result demonstrated that toxicity is present at concentrations greater than 5.0 ㎎/㎖. The protective effect of ASE on LPS and UVB-induced in the zebrafish was investigated. Intracellular reactive oxygen species(ROS) generated by the exposure of zebrafish to LPS, UVB-radiation were significantly decreased after treatment with ASE at 0.1 ㎎/㎖. As a result, ASE similarly reduced UVB-induced ROS generation and cell death in live zebrafsih. Therefore, it is suggested that ASE has anti-Inflammatory effects and can possibly be used as a functional substance for skin protection in the future.

목차

1. Introduction

2. Experiment

3. Results and Discussion

4. Conclusions

키워드

Ascidian shell Zebrafish Anti-Inflammation Ultraviolet B Toxicity Antioxidant