Optimization of ginseng hairy roots culture and its ginsenoside analysis

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Joong Gu Ji Sun Kyun Yoo
• 간행물 정보: 『한국응용과학기술학회지』한국유화학회지 제35권 제4호, 995~1002쪽, 전체 8쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

Hairy root culture of ginseng is industrially prospected because the cultivation period of ginseng is relatively long. In this study, the effect of medium concentration and sucrose concentration on hairy root culture of ginseng was evaluated. The optimization of ginseng hairy roots transformed by Agrobacterium rhizogene were performed liquid medium. The MS(Murashinge & Skoog basal medium) concentration was selected with 1/2 strength MS and the optimal sucrose concentration was determined at 2-3%(w/v). At the optimum culture condition, The yield (the ratio of weight of grown hairy root cultures to weight of fresh ginseng hairy roots) and production rate of ginseng root were 19.42 times and 5.73 g/l-day. The major ginsenosides were Rb group, Re and Rg1. The produced total ginsenoside content in the solid medium was 9.87 (mg/g) and increased 1.34 times in the liquid medium (13.23 mg/g). In solid culture, the contents of ginsenosides Rb, Re and Rg1 were 2.14, 3.65 and 1.87 mg/g, respectively. In liquid culture, the contents of ginsenosides Rb, Re and Rg1 were 3.54, 4.12 and 2.63 mg/g, respectively.

목차

Abstract

1. Introduction

2. Meterials and Method

3. Results and Discussion

4. Conclusion

References

키워드

ginseng hairy root optimization Agrobacterium rhizogene ginsenosides culture