감잎(시엽(枾葉))의 항산화 성분 분리

• 영문명: Separation of Antioxidant compounds from Persimmon Leaves
• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: 신두호
• 간행물 정보: 『한국응용과학기술학회지』한국유화학회지 제14권 제1호, 103~107쪽, 전체 5쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 1997.05.30

영문 초록

This study was carried out to separate and identify the antioxidative substances in persimmon leaves. The antioxidative substances in persimmon leaves were extracted by methanol. The extract was fractionnated by SEP-PAK cartridge colum. From these results five fractions(F-I${\sim}$ 수식 이미지V) were obtained. Antioxidative activity of each fractions was examined by the DPPH methord. The F-II, III and IV showed antioxidative activity and among them F-II and F-III showed the strongest. Five frations were separated by TLC using ethylacetate : chloroform : formic acid : $H_2O$ 수식 이미지(8 : 1 : 1 : 1 v /v) as the solvent. From these results were obtained spots of Rf 0.71, 0.35 and 0.25. This spots were scraped from the plate and extracted by methanol. The extracts thuse obtained were used for examination of identify by TLC, UV /VIS-spectrophotometer and HPLC. Among them spot of Rf 0.71 were demonstrated to catechin and the spots of Rf 0.35 and 0.25 was suggested to polyphenol substances.