Gas chromatographic and mass spectrometric analysis of conjugated steroids in urine

• 영문명: Gas chromatographic and mass spectrometric analysis of conjugated steroids in urine
• 발행기관: 군산대학교 수산과학연구소
• 저자명: JONG MAN YOON KYUNG HO LEE
• 간행물 정보: 『군산대학교 수산과학연구소 연구논문집』제5집, 86~93쪽, 전체 8쪽
• 주제분류: 농수해양 > 수산학
• 파일형태: PDF
• 발행일자: 2004.07.03

영문 초록

This study was carried out qualitatively and quantitatively to investigate the presence and the concentrations of anabolic steroids in urine collected from orally administered humans. Microanalysis of conjugated steroids by gas chromatography and mass spectrometry (GC/MS) has been carried out. Following oral administration three major metabolites of anabolic steroid drugs ,have been detected and partially characterized. The six steroids can be analysed at the same time in 17 min. The Lower detection limit was 10 ng/ml in 5 ml of urine. The conjugated steroids from urine were centrifuged to 2.430 g for 10 min. the supernatant solution passed through Amberlite XAD-2 column and the steroids eluted fraction esterified by using MSTFA and TMSI. The rate of metabolism and urinary excretion seem to be reasonably fast.

목차

Abstract

1. Introduction

2. Materials and methods

3. Results

4. Discussion

Acknowledgments

References