Chick early amniotic fluid attenuates lipopolysaccharide-induced acute lung injury

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Chengxin Yin Shan Lin Jianzhou Li Xiejiu Chen Borui Li Hui Yang Baiping Cui
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제29권 제6호, 713~725쪽, 전체 13쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2025.11.30

국문 초록

Acute lung injury (ALI) and acute respiratory distress syndrome are major causes of acute respiratory failure and even death. Acute pneumonia can be accompanied by damage to the alveolar epithelium. In vitro functional experiments were performed by constructing inflammatory models in human alveolar epithelial cells. It was found that the chick early amniotic fluid (ceAF) extracted from SPF-grade early chicken embryos could significantly reduce the inflammation of human lung epithelial cells (Calu-3) induced by lipopolysaccharide (LPS) and COVID-19 protein pseudovirus, and reduce the gene expression of inflammatory factors. Pneumonia is one of the most common risk factors for causing ALI. An acute pneumonia model was constructed by LPS inhalation. Tail vein injection of ceAF significantly improved inspiratory and expiratory resistance, enhanced lung compliance, and reduced death rate due to acute pneumonia in mice. Neutrophil recruitment is an important feature of both acute inflammation and ALI. HE staining and immunohistochemical staining showed that ceAF treatment significantly reduced lung inflammatory edema and neutrophil cell aggregation, and promoted the resolution of inflammation in ALI, while reducing the inflammatory response in the blood. Further mass spectrometry experiments revealed that nicotinamide adenine dinucleotide (NAD+), guanosine and deoxyinosine were the primary components of ceAF. While each compound partially alleviated LPS-induced acute pneumonia, their effects were slightly weaker than those of ceAF, suggesting that ceAF’s anti-inflammatory properties may arise from the synergistic action of these molecules.

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키워드

Amniotic fluid Inflammation Lipopolysaccharide Pneumonia