Precision proteomics with TurboID: mapping the suborganelle landscape

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Han Byeol Kim Kwang-eun Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제28권 제6호, 495~501쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2024.11.01

국문 초록

Recent research underscores the pivotal role of cellular organelles, such as mitochondria, the endoplasmic reticulum, and lysosomes, in maintaining cellular homeostasis. Their dynamic interactions are critical for metabolic regulation and stress response. Analysis of organelle proteomes offers valuable insights into their functions in both physiology and disease. Traditional proteomic approaches to studying isolated organelles are now complemented by innovative methodologies focusing on inter-organelle interactions. This review examines the integration of advanced proximity labeling technologies, including TurboID and split-TurboID, which address the inherent limitations of traditional techniques and enable precision proteomics of suborganelle compartments and inter-organellar contact sites. These innovations have led to discoveries regarding organelle interconnections, revealing mechanisms underlying metabolic processes such as cholesterol metabolism, glucose metabolism, and lysosomal repair. In addition to highlighting the advancements in TurboID applications, this review delineates the evolving trends in organelle research, underscoring the transformative potential of these techniques to significantly enhance organelle-specific proteomic investigations.

목차

INTRODUCTION

SINGLE ORGANELLE

ORGANELLE CONTACT

CONCLUSION

REFERENCES

키워드

Endoplasmic reticulum Lysosome Mitochondria Proteomics