저온저장 음나무 배발생 캘러스로부터 체세포배 유도와 식물체 재생

• 영문명: Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus
• 발행기관: (사)한국식물생명공학회
• 저자명: 이나념(Na Nyum Lee) 최용의(Yong Eui Choi) 문흥규(Heung Kyu Moon)
• 간행물 정보: 『Journal of Plant Biotechnology』42권 4호, 388~395쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.31

영문 초록

Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at 4℃ in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at 4℃ without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

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키워드

Cold storage Embryogenic callus Somatic embryo germination Kalopanax setemlobus Plant conversion