다양한 계통의 옥수수 미성숙배로부터 캘러스 유도와 식물체 재분화

• 영문명: Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L .)
• 발행기관: (사)한국식물생명공학회
• 저자명: 홍준기(Joon Ki Hong) 박기진(Ki Jin Park) 이강섭(Gang-seob Lee) 김둘이(Dool Yi Kim) 김주곤(Ju-kon Kim) 이승범(Seung Bum
• 간행물 정보: 『Journal of Plant Biotechnology』44권 1호, 49~55쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.03.31

영문 초록

We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/L, MES 0.5 g/L, Dicamba 1.5 mg/L, 2,4-D 0.5 mg/L, AgNO3 4 mg/L, and sucrose 20 g/L, showed the highest frequency of callus induction. The highest number of shoots regenerated when the embryogenic callus were transferred to MS medium supplemented with 5 mg/L zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/L indole-3-butyric acid (IBA). Additionally, under the same culture conditions, immature zygotic embryos from maize grown in the field also had a high frequency of plant regeneration. Except one genotype, 15 genotypes showed callus induction and shoot regeneration. Among the 16 genotypes tested, H99, B98, HW3, and B73 yielded the best plant regeneration. H99 showed maximum shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of the maize plant plays very important roles for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation.

목차

서 언

재료 및 방법

결과 및 고찰

사 사

References

키워드

Callus induction Embryogenesis Genotype Maize Molecular breeding Regeneration