윤판나물(Disporum sessile) 아배양에 의한 식물체 재분화에 영향하는 배지 종류 및 생장조절물질 효과

• 영문명: Effect of kinds of medium and plant growth regulators for plantlets regeneration by bud culture in Disporum sessile
• 발행기관: (사)한국식물생명공학회
• 저자명: 이나념(Na-nyum Lee) 김지아(Ji-ah Kim) 김태동(Tae Dong Kim) 김용욱(Yong Wook Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』44권 1호, 42~48쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.03.31

영문 초록

This study was conducted to establish the optimal condition for in vitro propagation of Disporum sessile. In the experiments with different kinds of media (MS, B5 and WPM) and explant types for shoot induction and elongation, the highest number of shoot inductions (2.5/explant) was shown when the axillary bud explants were cultured on MS medium without plant growth regulators (PGRs). The best shoot elongation (7.2 cm) was also obtained when the apical buds were cultured on MS medium. The effect of BA pulse treatments with in vitro shoots was also examined. The highest in shoot induction (2.29/explant) and elongation (7.28 cm) was observed when the shoots from axillary buds were cultured on the media without PGRs. However, it decreased with increasing duration of BA pulse-treatment. The highest rooting rate (100%) and number of root inductions (21.3/explant) were achieved with 1.0 mg/L indole-3-butyric acid (IBA) treatment, whereas no differences were observed by different shoot types. The regenerated in vitro plantlets were hardened and successfully established in soil.

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서 론

재료 및 방법

결과 및 고찰

References

키워드

Axillary bud BA pulse treatment Micropropagation Plant regeneration Shoot elongation