A STUDY ON THE EXPRESSION OF TYPE I AND TYPE II COLLAGEN GENES AND PROTEINS IN THE DEVELOPING HUMAN MANDIBLE

• 영문명: A STUDY ON THE EXPRESSION OF TYPE I AND TYPE II COLLAGEN GENES AND PROTEINS IN THE DEVELOPING HUMAN MANDIBLE
• 발행기관: 대한치과교정학회
• 저자명: Yoon-Ah Kook Sang-Cheol Kim Eun-Cheol Kim
• 간행물 정보: 『The Korean Journal of Orthodontics』제25권 제6호, 723~731쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.12.31

영문 초록

Type I and type II collagens are considered the major collagens of bone and cartilage respectively. Monitoring the patterns of those gene and protein expressions during development will provide a basis for the understanding of the normal and abnormal growths. This study was undertaken to investigate the expression of collagen genes and proteins involved in the developing human mandible. Fifty embryos and fetuses were studied with Alcian blue-PAS, Masson´s Trichrome, reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and Southern blot analysis. Our results showed that pro-α1(II) collagen gene expression begins in the 5th week. Type II collagen is synthesized in mesenchymal cells in advance of overt chondrogenesis. The gene expression for type II collagen was highest during the appearance of Meckel´s cartilage. There was a switch in collagen protein expression from type I to type II during the appearance stage of Meckel´s cartilage. The distribution of the mRNA for type II collagen corresponded well with the pattern of type II collagen protein. The endochondral ossification was observed where there was direct replacement of cartilage by bone.

키워드

Human Embryo and Fetus Mandible Meckel´ s Cartilage Collagen type I and II