EFFECTS OF SODIUM NITROPRUSSIDE ON THE FORMATION AND ACTIVATION OF THE OSTEOCLAST IN CULTURE

• 영문명: EFFECTS OF SODIUM NITROPRUSSIDE ON THE FORMATION AND ACTIVATION OF THE OSTEOCLAST IN CULTURE
• 발행기관: 대한치과교정학회
• 저자명: Young-Jae Yoo Jung-Kun Kim Kyung-Suk Cha
• 간행물 정보: 『The Korean Journal of Orthodontics』제25권 제6호, 705~714쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.12.31

영문 초록

Due to the great deal of effort that has gone into the study of osteoclastic differentiation and activation over the last few decades, the mechanisms of these two events have been discovered gradually. Nitric oxide(NO^-), which is produced from arginine by a nitric oxide synthase, opened up a new area of biological research. Recently, it has been reported that NO^- is produced by osteoblasts stimulated by lipopolysaccharide and several other cytokines. In this study, the effect of sodium nitroprusside(SNP), a donor of nitric oxide(NO^-), on osteoclast-like cell formation and on mature osteoclast function was examined. To determine the mechanism of the inhibitory effects of SNP decreased not only the basal ⁴⁵Ca release but also thee bone resorption induced by PTH and l,25-dihydroxyvitamin D₃ (I ,25[OH]₂D₃). The inhibitory effect of SNP on bone resorption induced by PTH appeared 2 days after treatment, whereas SNP effect on inhibiting bone resorption induced by l,25[OH]₂D₃ appeared at the third days. When chicken and rat osteoclasts were cultured on dentin slices, treatment of 300µM SNP resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and average individual area. We also examined the effect of SNP on formation of osteoclast-like cells that is TRAP-positive multinucleated cells from chicken and rat bone marrow cells in the presence or absence of 10^-8M l,25[OH]₂D₃. The addition of 300 µM SNP inhibited the formation of TRAP-positive multinucleated cells. The present data suggest that SNP, possibly as a NO^- donor, inhibits the osteoclastic differentiation and osteoclastic activity.

키워드

Sodium nitroprusside Osteoclast