Requirement of Pretone by Thromboxane A2 for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat

• 영문명: Requirement of Pretone by Thromboxane A₂ for Hypoxic Pulmonary Vasoconstriction in Precision-cut Lung Slices of Rat
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Su Jung Park Hae Young Yoo Hye Jin Kim Jin Kyoung Kim Yin-Hua Zhang Sung Joon Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제16권 제1호, 59~64쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2012.01.01

영문 초록

Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs (HPV-lung) can be triggered without pretone conditioning, we suspected that a putative tissue factor might be responsible for the pretone of HPV. Here we investigated whether HPV can be also observed in precision-cut lung slices (PCLS) from rats. The HPV in PCLS also required partial contraction by U46619. In addition, K^＋ channel blockers (4AP and TEA) required U46619- pretone to induce significant contraction of PA in PCLS. In contrast, the airways in PCLS showed reversible contraction in response to the K^＋ channel blockers without pretone conditioning. Also, the airways showed no hypoxic constriction but a relaxation under the partial pretone by U46619. The airways in PCLS showed reliable, concentration-dependent contraction by metacholine (EC₅₀, ∼210 nM). In summary, the HPV in PCLS is more similar to isolated PA than V/P lungs. The metacholine- induced constriction of bronchioles suggested that the PLCS might be also useful for studying airway physiology in situ.

키워드

Lung slice Hypoxic pulmonary vasoconstriction Thromboxane A₂ Airway smooth muscle