Isolation and identification of organic compounds-degrading bacterium, Bacillus atrophaeus YBK-W4, for the treatment of organic wastes

• 발행기관: 한국예방수의학회
• 저자명: Sung Bum Lee Ik Ho Choi Youn-Tae Chi Hu-Jang Lee
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.49, No.2, 51~57쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

In this study, a bacterial strain exhibiting high degradation activity toward organic wastes was isolated and taxonomicallyidentified. Five strains with excellent hydrolytic activity toward starch, lipids, and proteins were isolated from slaughter wasteprocessors. Among the five strains, YBK-W4, which showed the highest amylase, protease, and lipase activities, was selected.Through 16S rRNA sequencing, YBK-W4 was identified as Bacillus atrophaeus (B. atrophaeus) and designated B. atrophaeusYBK-W4. The optimal temperature and pH for enzyme activity of B. atrophaeus YBK-W4 were 37~42°C and pH 7, respectively.The bacterium was grown on Luria-Bertani (LB) agar containing 12% NaCl, where enzyme activity was 11.4~29.7% of thatobserved in LB agar without NaCl. These results suggest that B. atrophaeus YBK-W4 is a useful bacterial agent for thedecomposition of organic waste, such as food and slaughterhouse waste.

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organic waste degradation enzyme activity Bacillus atrophaeus YBK-W4