NaCl 스트레스에 따른 출수기 시기의 엽록소 측정을 활용한 밀 핵심집단의 전장유전체연관 분석

• 영문명: Genome-Wide Association Analysis of a Wheat Core Collection Based on Chlorophyll Content at Heading Stage in Response to NaCl Stress
• 발행기관: 한국육종학회
• 저자명: 민경도(Kyoung Do Min) 현도윤(Do Yoon Hyun) 박상용(Sang Yong Park) 김재윤(Jae Yoon Kim)
• 간행물 정보: 『한국육종학회지』Vol.57 No.2, 175~188쪽, 전체 14쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2025.06.01

국문 초록

본 연구는 밀의 이삭이 나오는 출수기에 염해 스트레스를 처리하고 유닛별 SPAD value 및 토양전기전도도를 활용한 STI지수를 기반으로 내염성 계통을 선발하고 GWAS 분석을 통해 염해 스트레스와 연관된 SNP 및 유용유전자 후보군을 탐색하였다. 1. 밀의 핵심집단내에 계통번호 cc1687과 cc1066 계통은 유닛2와 유닛3의 STI 지수에서 공통적으로 높은 값을 띄어 내염성 우수자원 후보로 선발하였음. 2. 전장유전체 연관분석(GWAS) 결과 3D, 5D, 7D에서 유의성이 있는 SNP를 발굴하였으며 발굴된 SNP는 AX-94929101, AX-94510535, AX-94411611, AX-AX-94615611 이며 각각 엑손, 3'UTR, 인트론영역에서 존재하였음. 3. 탐색된 SNP를 중심으로 250kbp를 탐색한 결과 TraesCS3D02G218100(AAA+ ATPase domain-containing protein), TraesCS3D02G218400 (C3HC-type domain-containing protein), TraesCS5D02G059500 (Zinc finger LSD1-type domain-containing protein), TraesCS7D02G233900 (Uncharacterized protein), TraesCS5D02G175000(Phosphoglycolate phosphatase), TraesCS5D02G175100 (GBF-interacting protein 1 N-terminal domain-containing protein)를 내염성 연관 candidate gene으로 선발하였음. 4. 선발 SNP의 핵심집단 내 하플로타입 분석을 통해 AX-94510535가 유전자형에 따른 표현형이 유의성이 나타났으므로 추후 STI 지수를 기준으로 내염성 계통을 선발할 마커로 활용이 가능할 것으로 사료됨.

영문 초록

Salt stress is a major abiotic factor that limits wheat production worldwide. However, this threat is increasing significantly because soil salinity affects approximately 20% of the irrigated agricultural land globally, leading to significant yield losses by impairing plant growth and photosynthetic efficiency. This study aimed to identify single-nucleotide polymorphisms (SNPs) associated with salt tolerance in wheat core collections during the heading stage under saline stress conditions. Chlorophyll content, a physiological indicator of salt tolerance at heading, and soil electrical conductivity (EC) were measured in 609 accessions and a Salt Tolerance Index (STI) was subsequently constructed. Genome-wide association studies (GWAS) were performed using a 35 K SNP chip to identify significant marker-trait associations. Three models (MLM, FarmCPU, and BLINK) were employed for the GWAS, with FarmCPU and BLINK demonstrating superior power over the MLM in controlling false positives. GWAS results revealed four significant SNPs (AX-94929101, AX- 94615611, AX-94510535, and AX-94411611) located on chromosomes 3D, 5D, and 7D. AX-94510535 exhibited significant phenotypic differences based on SNP genotype, suggesting its potential as a marker for STI. Furthermore, the identified candidate genes, TraesCS3D02G218100, TraesCS5D02G059500, and TraesCS5D02G175000, were implicated in biological processes such as DNA replication, cell death, and photosynthesis.

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키워드

wheat salt stress reproductive growth period GWAS breeding