Lorafocin, a mixture of Eunkyosan and alginate attenuate LPS-induced inflammation in RAW264.7 cells and inhibit growth of infectious bacteria

• 발행기관: 셀메드 세포교정의약학회
• 저자명: Bong-Keun Jang Hye-Yeon Kang Sunyoung Park Gun-Woo Lee Youngsun Kwon Sang-Eun Shin
• 간행물 정보: 『셀메드』Volume 14 Issue 14, 1~7쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2024.11.30

국문 초록

Influenza is an infectious disease which accompanies systematic inflammation. Although eliminating the primary or secondary infectious agent is an important action in treating influenza, it is still difficult because not only mutations of viruses but also antibiotics-resistance of bacteria. Eunkyosan, is traditionally used herbal formula to symptoms of influenza, have were reported for its pharmacological properties on inflammation. Also, Alginate, a physiologically active acidic polysaccharide obtained from brown algae, alleviates inflammation, exhibits anti-bacterial properties, and shows a collaborative therapeutic effect with an anti-bacterial agent for acute infections. Thus, we investigated anti-inflammatory effects of Lorafocin (a mixture of Eunkyosan and alginate) against lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cell line and its underlying mechanisms as well as anti-bacterial effects. In the RAW 264.7 macrophage cell line, Lorafocin treatment reduced the secretion of inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines induced by LPS. To elucidate the anti-inflammatory mechanism of Lorafocin, we investigated the involvement of the mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) signaling pathway. Studies demonstrated that Lorafocin exhibited anti-inflammatory efficacy by regulating Akt, c-Jun N-terminal kinase/stress-activated protein kinase (JNK), and extracellular signal-regulated kinase (ERK) 1/2 in LPS-induced RAW264.7 macrophages. Furthermore, Lorafocin inhibited the bacterial growth of Staphylococcus aureus and Escherichia coli. Taken together, Lorafocin may possess the capability to effectively modulate inflammatory responses and inhibit the growth of pathogenic bacteria.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULT

DISCUSSION

ACKNOWLEDGEMENT

CONFLICT OF INTEREST

REFERENCES

키워드

Lorafocin Eunkyosan Alginate Anti-inflammation Anti-bacteria