Practices in cryo-EM single particle analysis data processing: data transfer between RELION and cryoSPARC

• 영문명: Practices in cryo-EM single particle analysis data processing: data transfer between RELION and cryoSPARC
• 발행기관: 한국구조생물학회
• 저자명: Jinwoo Kim Jin Young Kang
• 간행물 정보: 『Biodesign』Vol 12, No 2, Jun, 29~38쪽, 전체 10쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2024.06.30

국문 초록

The recent advancement of cryo-EM data analysis software packages substantially improved the results, producing higher-resolution maps and finding hidden structural elements or conformations. Among many cryo-EM data analysis software, RELION and cryoSPARC are the two most used packages because they are easy to use, the GUI is intuitive, and produce good results. In this report, we compared the two packages regarding their functionalities and suggested practical methods to transfer data between the two packages using PyEM script, csparc2star.py. The protocols we suggested here would help cryo-EM users to utilize the different and diverse functions of the two packages in a variety of ways.

목차

INTRODUCTION

PART 1: COMPARISON BETWEEN RELION AND CRYOSPARC

PART 2: PRACTICES IN DATA TRANSFER BETWEEN RELION AND CRYOSPARC

CONCLUSION

GLOSSARY

ACKNOWLEDGEMENTS

REFERENCES