Efficient Cryopreservation of in vitro Grown Shoot Tips of Pear (Pyrus spp.) by Droplet-vitrification

• 영문명: Efficient Cryopreservation of in vitro Grown Shoot Tips of Pear (Pyrus spp.) by Droplet-vitrification
• 발행기관: 한국자원식물학회
• 저자명: 송재영(Jae-Young Song) 배진주(Jinjoo Bae) 한지원(Ji-Won Han) 고호철(Ho Cheol Ko) 이호선(Ho-Sun Lee) 남성희(Sung-Hee Nam) 이정로(Jung-Ro Lee) 윤병현(Byeong Hyeon Yun) 김금선(Keumsun Kim) 원경호(Kyungho Won) 신일섭(Il Sheob Shin)
• 간행물 정보: 『한국자원식물학회지』제36권 제6호, 571~579쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehyd-ration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for ‘Bartlett’ cultivar and 68.9% for ‘BaeYun No.3’ cultivar. Consequently, apical shoot tips of two pear cultivars, ‘Bartlett’ (P. communis) and ‘BaeYun No.3’ (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

Conflicts of Interest

References

키워드

Cryopreservation Droplet-vitrification Pyrus Regrowth