Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media

• 영문명: Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media
• 발행기관: 한국구조생물학회
• 저자명: Pil-Won Seo Jeong-Sun Kim
• 간행물 정보: 『Biodesign』Vol 10, No 4, Dec, 64~68쪽, 전체 5쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2022.12.31

영문 초록

Fructose-6-phosphate aldolase 1 (FSA) from Aeromonas media (AmFSA) is a class I aldolase characterized by the formation of a Schiff-base intermediate between a conserved lysine residue and a carbonyl substrate. FSA has shown high stereoselectivity and broad substrate spectrum. To provide a structural background for engineering as a catalyst for desired reaction product, AmFSA was cloned and expressed. The purified protein was crystallized from the precipitant of 0.1 M sodium acetate (pH 5.0), 1.4 M ammonium sulfate, and 10 mM β-mercaptoethanol. Diffraction data were collected to 2.1 Å resolution. The crystal belonged to the orthorhombic space group C2221 with unit-cell parameters a = 93.80 Å, b = 175.27 Å, c = 150.99 Å, and α = β = γ = 90°. The spatial positions of the five protein molecules in the asymmetric unit were determined by molecular replacement using the sequentially related Escherichia coli FSA structure.

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INTRODUCTION

RESULTS AND DISCUSSION

METHODS

REFERENCES