Plantlet Regeneration and PLBs Propagation of Bulbophyllum auricomum Lindl.

• 영문명: Plantlet Regeneration and PLBs Propagation of Bulbophyllum auricomum Lindl.
• 발행기관: 한국자원식물학회
• 저자명: 땡기아웅(Win Theingi Aung) Thang Tung Lian Zaw Phyo Aung Keuk Soo Bang Seung Hwa Baek
• 간행물 정보: 『한국자원식물학회지』제35권 제4호, 508~514쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.08.30

영문 초록

Bulbophyllum auricomum Lindl. is very popular among orchid growers due to the attractive fragrance of its flowers and has become an endangered orchid in Myanmar. In this study, we carried out an aseptic technique that can be used to conserve this endangered orchid species. The seeds of B. auricomum Lindl. were obtained from artificial pollination and cultured in MS basal medium for seed germination. The effect of coconut water and BAP in MS basal medium on callus induction was investigated. The highest callus induction was found at 2.0 ㎎/L BAP. The maximum growth of protocorm-like bodies (PLBs) was evaluated, and the best response was observed on MS medium supplemented with 150 mL/L coconut water at pH 5.6. MS basal medium supplemented with 150 mL/L coconut water along with 2.0 ㎎/L BAP and 1.0 ㎎/L NAA (MCBN) showed the highest number of plantlets at 15℃ at the second week of culture. At the second and third week of culture, MS medium supplemented with 2.0 ㎎/L BAP and 1.0 ㎎/L NAA (MBN) showed the best result in terms of the number of leaves and the longest leaves at 15℃ and 25℃, respectively. The present study showed evidence of successful in vitro propagation of B. auricomum Lindl.

목차

Introduction

Materials and Methods

Results

Discussion

Acknowledgments

Conflicts of Interest

References

키워드

Bulbophyllum auricomum Lindl. Coconut water pH Suspension culture Temperature