Evaluation of Yield Components from Transgenic Soybean Overexpressing Chromatin Architecture-Controlling ATPG8 and ATPG10 Genes

• 발행기관: 한국육종학회
• 저자명: Hyun Suk Cho Dong Hee Lee Ho Won Jung Seon-Woo Oh Hye Jeong Kim Young-Soo Chung
• 간행물 정보: 『Plant breeding and biotechnology』Vol.7 No.2, 34~41쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.06.01

영문 초록

AT-hook proteins are known to co-regulate transcription of genes through the modification of chromatin architecture. In plants, many genes encoding AT-hook proteins have been shown to be associated with increased seed yield or delayed senescence. In this study, we produced transgenic soybean plants overexpressing chromatin architecture-controlling ATPG8 and ATPG10 genes by Agrobacterium-mediated transformation and examined their agronomic traits to identify the yield increase in soybean crop similar to those seen in model plants, Arabidopsis. A total of 16 (3 of pB2GW7.0-ATPG8 and 13 of pCSEN-ATPG10 transformed) transgenic soybean plants were produced and their T1 seeds were harvested. Healthy and well-grown transgenic lines were selected (lines #1 and #2 from pB2GW7.0-ATPG8, and lines #8 and #9 from pCSEN-ATPG10), and the insertion and transcription level of genes were confirmed by PCR and RT-PCR with expected size. Investigation on agricultural traits confirms the increase in yield, plant height, the number of pods, and total seed weight with statistical significance when compared to wild-type soybean plants. The yield component study suggested that overexpression of ATPG8 and ATPG10 genes conferred positive effect on yield in transgenic soybean.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Soybean Chromatin architecture ATPG8 ATPG10 Agrobacterium-mediated transformation High-yield