Immature Persimmon (Diospyros kaki Thunb.) Ethanol Extract Inhibits Adipogenesis through AMP-Activated Kinase in 3T3-L1 Preadipocytes

• 발행기관: 한국자원식물학회
• 저자명: Seon-A Yoon Hyejin Hyeon Ho Bong Hyun Sung Chun Kim Boram Go Soyeon Oh Ji-Gweon Park Young-min Ham
• 간행물 정보: 『한국자원식물학회지』제38권 제3호, 275~283쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

We previously reported the anti-obesity effects of immature persimmon ethanol extract by analyzing changes in body weight, visceral fat weight, blood biochemicals, and fat production-related gene expression levels in a high-fat diet-induced obese mouse model. Therefore, this study investigated whether the 50% ethanol extract of Diospyros kaki Thunb. regulated lipid metabolism by identifying its mechanism in 3T3-L1 cells. As a result, it did not influence cell viability or toxicity, and a significant decrease in lipid droplet accumulation was observed in a concentration-dependent manner during adipogenesis (adipogenic differentiation). Consistent with the animal studies, Diospyros kaki Thunb. extract (in a dose-dependent manner) suppressed peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding proteins, which induce adipocyte differentiation in 3T3-L1 preadipocytes. In addition, it increased the levels of phosphorylated AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase in a dose-dependent manner in 3T3-L1 cells. Finally, we confirmed that sterol regulatory element-binding protein-1c is regulated by AMPK phosphorylation. These results indicated that immature persimmons (Diospyros kaki Thunb.) may serve as a natural agent for the prevention or treatment of obesity via the AMPK pathway.

목차

Introduction

Materials and Methods

Results

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Reference

키워드

Adipogenesis AMP-activated protein kinase phosphorylation Diospyros kaki Thunb. High-fat diet Protein kinase