Lactobacillus leichimanii D-Lactate Dehydrogenase 클로닝과 분리정제 및 Lactate Chirality 정성 정량 분석

• 영문명: Cloning and Purification of Recombinant Lactobacillus leichimanii D-Lactate Dehydrogenase and its Use in the Quantification of Lactate Chirality
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 추동원(Dongwon Choo)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제26권 2호, 97~102쪽, 전체 6쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2021.06.30

영문 초록

The D-lactate level in body fluids is an important factor associated with various metabolic diseases. Nevertheless, it is difficult to quantify lactate isomers in clinical laboratories. Here, a simple enzymatic assay employing recombinant D-lactate dehydrogenase to assess the level of D(-)-lactate in plasma or serum has been described. The D-lactate dehydrogenase gene from Lactobacillus leichmannii was cloned and expressed using E. coli expression system. The purified enzyme showed enantioselective activity, and D-lactate concentration was measured by estimating the level of NADH, a by-product of the enzymatic reaction. This rapid and accurate enzymatic assay can be applied to evaluate patients suffering from metabolite-related diseases.

목차

Ⅰ. 서 론

Ⅱ. 실 험

Ⅲ. 실험결과 및 고찰

Ⅳ. 결 론

키워드

복제 D-젖산 탈수소효소 D-젖산 대장균발현체계 분자비대 칭성 특이성 활성 Cloning D-Lactate Dehydrogenase(D-LDH) D-Lactate E. Coli Expression System Chirality-Specific Activity