Differentiation and Detection of Phytoplasma using PCR from Diseased Plant in Korea

• 발행기관: 한국자원식물학회
• 저자명: Kui-Jae Lee
• 간행물 정보: 『Plant Resources』Vol.3 No.3, 173~178쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2000.12.31

영문 초록

This test checked jujube witches -broom disease, sumac witches -broom disease, paulonia witches - broom disease, and mulberry dwarf disease whether or not they were infected by phytoplasma, using universal and specific primers. Upon treatment of DNA amplified by PCR of phytoplasma with Alu 1, Hpa I and Sal I restricted enzymes, distinction of phytoplasmas was possible. Particularly, phytoplasma of each host was distinguishable by treatment of Hpa I restricted enzyme. Meanwhile, analysis of restricted enzymes of jujube witches -broom disease showed a higher infectivity of phytoplasmas of two origins. There were a lot of relations between jujube witches -broom disease and sumac witches -broom disease, and between paulonia witches -broom disease and mulberry dwarf disease.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

Phytoplsama PCR RFLP witches -broom disease.