Plant Regeneration from Anther Culture of Panax ginseng

• 발행기관: 한국자원식물학회
• 저자명: Hee-Young Lee Altanzul Khorolragchaa Myung-Suk Sun Young-Joon Kim Yu-Jin Kim Woo-Seang Kwon Deok-Chun Yang
• 간행물 정보: 『한국자원식물학회지』제26권 제3호, 383~388쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.06.30

영문 초록

The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at 4℃ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog`s (MS) medium, 4.53 μM of 2.4-dichlorophenoxyacetic acid and 4.44 μM of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 μM of gibberellic acid and rooted in Gamborg`s B5medium supplemented with 14.7 μM of indole-3-butyric acid.

목차

Introduction

Materials and Methods

Results

Discussion

Acknowledgment

Literature Cited

키워드

Anther Panax ginseng Cold pretreatment Plant growth regulators Carbohydrates