물레나물 약배양에 의한 부정 신초 및 식물체 재분화

• 영문명: Adventitious Shoot and Plant Regeneration from AntherCulture of Hypericumascyron L.
• 발행기관: 한국자원식물학회
• 저자명: 고정애(Jeong Ae Ko) 김현순(Hyun Soon Kim) 김형무(Hyung Moo Kim)
• 간행물 정보: 『한국자원식물학회지』제21권 제5호, 368~373쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.10.31

영문 초록

In order to investigate the effects of low temperature pretreatment of floral bud and plant growth regulators on anther-derived callus and shoot differentiation, anthers were cultured on 1/2 MS medium supplemented with 2,4-D, NAA, BA and TDZ. This plant depends on the plant growth regulators, for these anthers couldn’t respond on 1/2 MS medium without plant growth regulators. 2,4-D was a prerequisite substance in this experiment, especially 52.6% of callus formation on MS medium with 2.0 mg/L 2,4-D alone. However, the optimum medium was on 1/2 MS medium with 0.1 mg/L 2,4-D and 1.0 mg/L BA for continuous growth and shoot differentiation from the anther. Calli derived from on MS medium with 2.0 mg/L 2,4-D transferred to the 1/2MS medium with TDZ and BA. TDZ were less superior to BA, only one anther could produce shoot onMS media with 1.0 mg/L TDZ. On the other hand, when the calli transferred to the medium with 3.0 mg/L BA, adventitious shoots were proliferated, subsequently, regenerated shoots elongated from the embryogenic calli. After floral buds of one week before anthesis were incubated at 5℃refrigerator for eight or fifteen days, anthers seperated from floral buds were cultured on 1/2 MS medium supplemented with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Callusing and shoot differentiation on anthers from treated at 5℃for eight days were more effective than those of fifteen days or control.

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재료 및 방법

결과 및 고찰

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인용문헌

키워드

anther-derived callus adventitious shoots 2,4-D NAA BA TDZ